This plasmid doesn't contain any promoter for the eukaryotic cells. The user can construct their own expression plasmid bearing a suitable promoter by transferring this gene. Be careful not to produce unexpected N-terminal fusion protein since this gene doesn't contain a STOP codon in front of start Met site.
>NLS-SCre ATGGTGCCTAAGAAAAAGAGGAAGGATTACAAAGATGATGATGATAAATC CCTTCTGACTACCAATAATCACAGTGTGGCTCTGTCATATGGTGAGCCTC CTTCAACACTTAATGACTCTCTGAAGGACAGTTACCAGAGATCTACTGAT GAGCTCCAGGCCCTCCTGTCCAAGCCACTGGCCCAGCTGACTGATGCAGA CAAGCTCAGGATCAGAGAAATTACTCAGGCCAAATTGAAGCACTTCCTGG ATAATGGCCACAGAACCAGAAGGGCTAACACTTGGAGAGCCCTGATGTCT AGGTGGGCAAAGTTTGAGTCCTGGTGTCTGACCAATAATTTGACCCCCCT CCCTGCCACCCCTGAAGTGGTGGCTACCTTCATTGAGTACTACCAGGCCT CAAGTTACACCACACTGAGCCAGTATGCCTGGGCTATTAACTCTTTCCAT GTGGAGTGTGGCCTTTTGTCCCCTGTTAGTTCCAAGACAGTGCAGGACAA GCAGAATGAAATCAGGATTGTGAAATTGGAGTCAGGGGGATTGGCCCAGG AGCAGGCTACTCCTTTTAGGTTGCACCACCTCCAGATGCTCATTGAGAGC TATGGAGAGTCTGAGAGGCTCCTGGACAAAAGAAACCTGGCACTGCTGAA CATTGCTTATGAGTCCCTCTTGAGGGAGTCAGAGCTGCTCAGAATCAAAG TGGGCCACTTGAAAAGCACTTTTGAGGGGGACTATGTGCTCTCTGTCCCT TACACCAAGACCAATGACTCTGGAGAGGAGGAGGTGGTGAACATCACTCC TCTGGGCTTCAAGCTGATCCAGAGGTACATCCAGGGAGCAGGTCTCACTA AGGAGGACTATCTGTTTCAGCCCATTGGGAGATCTAATAAGGTCAGTGTC CAGGCCAAGCCCATGAGCACTAGGACAGTGGACAGAGTCTTCCTGTGGGC CTTTGAGTCTCTGGGTATTGACAGACACTCTGCTTGGTCTGGACACTCTG CTAGAATTGGAGCAGCACAAGATCTTCTGGCAGCTGGCTATAGCATTGCT CAGATCCAGGAGAATGGCAGGTGGAAGTCTCCCATGATGGTCTTGAGATA TGGAAAGGACATTAAGGCTAAGGAATCAGCCATGGCTAAAATGTTGGCTG AAAGAAGGTAA
>pUCNLS-SCre TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCG GAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCG TCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATG CGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATA CCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATT CAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTAT TACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTA ACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATT CGAGCTCGGTACCTCGCGAATGCATCTAGATCCAATGAATTCGGCTTGAT CGAAGCTTGCCCACCATGGTGCCTAAGAAAAAGAGGAAGGATTACAAAGA TGATGATGATAAATCCCTTCTGACTACCAATAATCACAGTGTGGCTCTGT CATATGGTGAGCCTCCTTCAACACTTAATGACTCTCTGAAGGACAGTTAC CAGAGATCTACTGATGAGCTCCAGGCCCTCCTGTCCAAGCCACTGGCCCA GCTGACTGATGCAGACAAGCTCAGGATCAGAGAAATTACTCAGGCCAAAT TGAAGCACTTCCTGGATAATGGCCACAGAACCAGAAGGGCTAACACTTGG AGAGCCCTGATGTCTAGGTGGGCAAAGTTTGAGTCCTGGTGTCTGACCAA TAATTTGACCCCCCTCCCTGCCACCCCTGAAGTGGTGGCTACCTTCATTG AGTACTACCAGGCCTCAAGTTACACCACACTGAGCCAGTATGCCTGGGCT ATTAACTCTTTCCATGTGGAGTGTGGCCTTTTGTCCCCTGTTAGTTCCAA GACAGTGCAGGACAAGCAGAATGAAATCAGGATTGTGAAATTGGAGTCAG GGGGATTGGCCCAGGAGCAGGCTACTCCTTTTAGGTTGCACCACCTCCAG ATGCTCATTGAGAGCTATGGAGAGTCTGAGAGGCTCCTGGACAAAAGAAA CCTGGCACTGCTGAACATTGCTTATGAGTCCCTCTTGAGGGAGTCAGAGC TGCTCAGAATCAAAGTGGGCCACTTGAAAAGCACTTTTGAGGGGGACTAT GTGCTCTCTGTCCCTTACACCAAGACCAATGACTCTGGAGAGGAGGAGGT GGTGAACATCACTCCTCTGGGCTTCAAGCTGATCCAGAGGTACATCCAGG GAGCAGGTCTCACTAAGGAGGACTATCTGTTTCAGCCCATTGGGAGATCT AATAAGGTCAGTGTCCAGGCCAAGCCCATGAGCACTAGGACAGTGGACAG AGTCTTCCTGTGGGCCTTTGAGTCTCTGGGTATTGACAGACACTCTGCTT GGTCTGGACACTCTGCTAGAATTGGAGCAGCACAAGATCTTCTGGCAGCT GGCTATAGCATTGCTCAGATCCAGGAGAATGGCAGGTGGAAGTCTCCCAT GATGGTCTTGAGATATGGAAAGGACATTAAGGCTAAGGAATCAGCCATGG CTAAAATGTTGGCTGAAAGAAGGTAAGAATTCATTGGATCGGATCCCGGG CCCGTCGACTGCAGAGGCCTGCATGCAAGCTTGGCGTAATCATGGTCATA GCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATAC GAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAA CTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCT GTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTT TGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCG GTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACG GTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTC CATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCA GAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATAC CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACG CTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTAT CGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGC CACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGT ATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTC TTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA AGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATC TTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT TTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATT AAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCT GACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCT ATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGA TACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAC CCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAG GGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTA TTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTG CGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTT TGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACAT GATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATC GTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGC ACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGA CTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCG AGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAG AACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCT CAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCA CCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGC AAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGA AATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTAT CAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAA TAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACG TCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATC ACGAGGCCCTTTCGTC