You can search for clones by gene name, GenBank accession No., or keyword(s) in this page. In addition, advanced keyword search and homology search are also available.

M type clones ('M' attached at the end of the Product ID, or PID) are custom-made clones by modifying the clones we have into a full-length form.
E type clones ('E' attached at the end of the PID) are custom-made full-length ORF clones based on the public database sequences.
They will be newly constructed upon request.

At present, M type and E type clones are not available for requests from overseas countries.

The price depends on the clone type. This table lists the clone types and their price.

Standard clones (PIDs without E or M) will be delivered within two weeks in Japan or two to four weeks to overseas countries. As for E type or M type clones, we will estimate the time needed for delivery upon request. Please contact us at flexiclone at kazusagt.com.

Yes, it is possible. As described above, such clones are classified as E type clones. We will estimate the price and delivery time for E type clones upon request. Please contact us at flexiclone at kazusagt.com.

The original clones are derived from various sources: SNPs or splicing variants derived from the same genetic locus are included. Therefore, one gene symbol sometimes corresponds to several sequences/clones.

Some of the ORFeome clones are provided in two forms, namely with or without a stop codon. The latter is to facilitate easy production of C-terminal fusion proteins. Each variant clone possesses a different PID. Please decide a most suitable clone(s) for your research. We advise you to check the nucleotide sequence that is linked with the PID before your order.

They are clones derived from a single gene locus to which multiple gene symbols have been assigned. For example, KIAA0001 is also known as GPR105, P2RY14 or P2Y14. Therefore, when you search for KIAA0001, you will obtain results for P2RY14 as well. At our web site, we use the gene symbols of the original clone resources.

Yes, we have conformed the full-length sequences of Kazusa original clones (PIDs beginning with ORK).
The OC clones (PIDs beginning with ORS/ORH) have been conformed by the ORFeome Collaboration.

Yes, we have conformed the full-length sequence at either one of the steps of cloning, especially when PCR products are to be inserted into pF1K or pFN21A vectors. This is because cloning using PCR results in high frequency of mutation. In contrast, cloning using a restriction enzyme or by homologous recombination in E. coli are considered to be of low frequency of mutation. Thus, we sequence only both ends of the insert and check the insert size.

Usually we dispatch 100 ng of clone DNA per order dissolved in 10μl TE solution in a 500μl tube. It will be sent out at the ambient temperature. To store the DNA for a long term, please keep the package frozen at -20℃.

Yes we do. After constructing Flexi HaloTag Type clones, their expression has been tested in HEK293 cells using the HaloTag TMR ligand. Fusion proteins were observed by fluorescence microscopy and then by SDS-PAGE. You can obtain detailed information in each clone page that is linked with the PID in the search result.

It depends on the ORF sequence. Flexi clones have one of the flanking sequences shown below.

Yes we do. You will receive a 10 % discount by ordering 20-49 clones and a 20 % discount by ordering more than 50 clones. If you would like to obtain this quantity discount, please send your order by e-mail to: flexiclone at kazusagt.com, instead of performing the ordering process on our web page.
Please note that quantity discount is NOT applicable for M type and E type clones.

Please refer to the introduction of the vector (GenBank Accession No. EU621374). The ORFs are inserted at the Barnase gene region.